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Instruments

 

CELLULAR IMAGING

 INSTRUMENT  OPERATOR  DESCRIPTION AND RESOURCES

Confocal Microscope LSM 710
                              
Christophe KLEIN

6 excitation wave length: 405, 458, 488, 514, 561 et 633nm.
Spectral unmixing: detects up to 10 parameters simultaneously.
Application


Fluorescence microscope Axiovert 200M

Christophe KLEIN

4 detection filters: DAPI (Ex=365nm; Em=445nm), FITC (Ex=470nm; Em=525nm), TRITC (Ex=545nm; Em=605nm), Cy5 (Ex=640nm; Em=690nm)
Application

 

Slide Scanner

 

Christophe KLEIN

Slide scanner ZEISS Axio Scan Z1

  • Slide digitalization x10, x20,  x40
  • Bright field (histochemistry and Immunohistochemistry)
  • Fluorescence (Up to 5 markers)
  • Virtual image processing with Zen software
  • Image analysis (cell counting, area measurement with Visiopharm software)

Laser Microdissection Leica AS-LMD

Christophe KLEIN

Dissection specific subpopulations of tissue cells Under direct microscopic visualization

Cryostat Leica CM 3050S

Christophe KLEIN

Located next to the laser microdissector to be used in conjunction with it.

 

FLOW CYTOMETRY

 INSTRUMENT OPERATOR DESCRIPTION AND RESSOURCES 


Luminex 

                                        
Kariman CHABA
Hélène FOHRER-TING
Estelle DEVEVRE

Rapid measurment of maximum 500 analytes per sample simultaneously with  96 and 384 well plates.
Application

 Bench top cytometer BD LSR II Hélène FOHRER-TING
Estelle DEVEVRE  
3 lasers, 10 parameters. Uses 12x75mm polystyrene FACS tubes. ref 352054.

Description
Optics
Application

 
Cytometer Gallios

Hélène FOHRER-TING
Estelle DEVEVRE 

 
3 lasers, 10 paramètres.
32 tubes carousel
Optics                                                                        
Application
 

 

FACS Aria III 

 

Hélène FOHRER-TING
Estelle DEVEVRE 

16 parameters, 4 lasers. Sort up to 4 populations.

Description
Optics
Application

 

BD Influx

Hélène FOHRER-TING
Estelle DEVEVRE

17 parameters, 4 lasers. Sort up to 6 populations.
Sorted sample uses exclusively polypropylene FACS tubes FALCON ref 352063

Description
Optics
Application 


 Automacs

Hélène FOHRER-TING
 

Magnetic cell sorting. Cell labelling, automated positive and negative cell selection. Increased column capacity compared to manual selection. 
 
Application   


 ELISPOT
 
Estelle DEVEVRE

ELISPOT. Single and double color.

Application

 

 

DATA ANALYSIS

 INSTRUMENT OPERATOR DESCRIPTION AND RESSOURCES 


Flow Cytometry

                                        
Hélène FOHRER-TING
Estelle DEVEVRE

We can provide you with a complete flow cytometry data analysis service . From raw data to final figures for publication
Softwares available: DIVA, Flow Jo, Spice, Cytobank

Cell Imaging Christophe KLEIN We can provide you with a complete cell imaging data analysis service. From raw data to final figures for publication
Softwares available: Image J, Visopharm, Zen

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