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Equipment

 

HISTOLOGY

 Equipment  Staff in charge  Description and resources
Laser Microdissection
Leica AS-LMD

                                              Christophe KLEIN

Dissection of specific subpopulations of tissue cells under direct microscopic visualization

Cryostat
Leica CM 305S

Kevin GARBIN

Frozen block sectioning. Sections from 5µm thickness

 Microtome
Leica RM2145

 Kevin GARBIN

Paraffin block sectioning. Sections from 3µm thickness 

Paraffin embedding
Leica ASP300 and HistoCore Arcadia

Kevin GARBIN

Automated Dehydration and impregnation of fixed and bon-fixed tissue samples.
Autostainer
Ventana Roche Discovery XT

Kevin GARBIN

Capacity of 30 (frozen or paraffin) slides that can be treated independently for IHC, IF, FISH application.

 Autostainer DAKO 48L
Kevin GARBIN 
Capacity of 48 (frozen or paraffin) slides that can be treated independently for IHC, IF, FISH application. unmasking and dewaxing treatments must be done externally prior to use the equipment. A TintoRetriever Digital Pressure Cooker with Thermometer is available to perform unmasking and dewaxing
 Autostainer
Leica Bond RX
 Kevin GARBIN Automated autostainer suitable for IHC, ISH, FISH, multiplexing experiments. Slides can be treated independently

   

 

CELLULAR IMAGING

 Equipment  Staff in charge  Description and resources

Confocal Microscope 
Zeiss LSM 710
                              
Christophe KLEIN

6 excitation wave length: 405, 458, 488, 514, 561 et 633nm.
Spectral unmixing: detects up to 10 parameters simultaneously.
Application


Fluorescence microscope
Zeiss Axiovert 200M

Christophe KLEIN
Equiped with 4 detection filters: DAPI (Ex=365nm; Em=445nm), FITC (Ex=470nm; Em=525nm), TRITC (Ex=545nm; Em=605nm), Cy5 (Ex=640nm; Em=690nm)
Application

 

Slide Scanner Zeiss
Axio Scan Z1

 

Christophe KLEIN

  • Digitalization x10, x20,  x40
  • Automated brightfield and multichannel fluorescence (up to 5 biomarkersk, or 4 biomarkers nuclei staining)
  • coupled with Zen software 

Image analysis can be performed with Visiopharm software (-cell counting, measure of areas of cells…). Analysis workstations available.

Laser Microdissection Leica AS-LMD Christophe KLEIN Dissection specific subpopulations of tissue cells Under direct microscopic visualization
Spectral microscope Perkin Elmer
Mantra

Christophe KLEIN

Can acquire um to 7 fluorochromes or 4 immunohistochemical stains.
Distinctly differentiate autofluoresence.
Perkin Elmer InForm worstation allows automatic cell recognition and couting function of their phenotype.
Application

 ELISPOT
CTL Immunospot


Estelle DEVEVRE 
 Enzymatic and fluorescence detection up to 2 colors
Application

 

FLOW CYTOMETRY

 Equipment Staff in charge Descritopn and resources

Multi-analyte assay
Luminex
FlexMap3D 

Hélène FOHRER-TING
Estelle DEVEVRE

Rapid measurment of maximum 500 analytes per sample simultaneously with  96 and 384 well plates.
Application

 
Bench top cytometer BD LSR II


Hélène FOHRER-TING
Estelle DEVEVRE  
10 parameters on 3 lasers. Compatible with 12x75mm polystyrene FACS tubes. ref 352054.

Description
Optics
Application

 FACS cell sorter
BD
Aria III 

Hélène FOHRER-TING
Estelle DEVEVRE 

16 parameters on 4 lasers. Sort up to 4 populations simultaneously

Complete description
Optics
Application

 

FACS cell sorter
BD Influx

Hélène FOHRER-TING
Estelle DEVEVRE

17 parameters on 4 lasers. Can sort up to 4 populations simultaneously. Compatible with 12x75mm polypropylene FACS tubes (Ref. 352063)

Complete description
Optics
Application 


 Automated magnetic cell sorter Miltenyi Biotec Automacs Pro

Hélène FOHRER-TING
 

Fully automated cell labeling, and magnetic cell sorting. Can program up to 6 independent sorting. Doubled column capacity compared to manual selection. 
 Application   

 

SERVICES

 TECHNOLOGY OPERATOR DESCRIPTION AND RESSOURCES 

Multi-analyte assay Luminex

Hélène FOHRER-TING
Estelle DEVEVRE

More sensitive tha ELISA, Luminex technology is the right choice. When no satisfying commercial solution is available, we can help you set up an assay. 


Flow Cytometry 
 
Hélène FOHRER-TING
Estelle DEVEVRE

We can provide you with a complete flow cytometry data analysis service . From raw data to final figures for publication
Softwares available: DIVA, Flow Jo, Spice, R
Application

 
Cell Imaging Christophe KLEIN We can provide you with a complete image analysis service. From raw data to final figures for publication
Softwares available: Image J, Visopharm, Zen

 

 

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